Membrane imaging by second-harmonic generation microscopy
نویسندگان
چکیده
منابع مشابه
Membrane imaging by simultaneous second-harmonic generation and two-photon microscopy: errata.
We demonstrate that simultaneous second-harmonic generation (SHG) and two-photon-excited fluorescence (TPEF) can be used to rapidly image biological membranes labeled with a styryl dye. The SHG power is made compatible with the TPEF power by use of near-resonance excitation, in accord with a model based on the theory of phased-array antennas, which shows that the SHG radiation is highly structu...
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We describe a novel second harmonic generation (SHG) microscope that employs heterodyne detection by interfering the epi directed SHG from a sample with SHG from a reference crystal. In addition, the microscope provides complementary reflectance information based on optical coherence microscopy (OCM). The instrument features dual balanced detection to minimize the effect of source fluctuations,...
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Second-harmonic generation (SHG) can be used for imaging membrane potential in neurons, but poor signal-to-noise (S/N) limits accurate measurements of small voltage transients. We use photon counting to improve the S/N of weak SHG signal detection. Photon counting generates shot-noise limited and integrable signals, eliminates pulse-to-pulse variation, and built-in discriminators reduces the ba...
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Nonlinear microscopy has become an important tool for imaging biological samples such as neurons. In two photon fluorescence, a fluorophore absorbs two photons at a low energy state, one of its electrons briefly converts to a higher energy state, and it emits a single excited photon at an energy level higher than that of the absorbed photons. In second harmonic generation (SHG), laser light is ...
متن کاملSecond-harmonic imaging microscopy of living cells.
Second harmonic generation (SHG) has been developed in our laboratories as a high-resolution nonlinear optical imaging microscopy for cellular membranes and intact tissues. SHG shares many of the advantageous features for microscopy of another more established nonlinear optical technique: two-photon excited fluorescence (TPEF). Both are capable of optical sectioning to produce three-dimensional...
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ژورنال
عنوان ژورنال: Journal of the Optical Society of America B
سال: 2000
ISSN: 0740-3224,1520-8540
DOI: 10.1364/josab.17.001685